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Class 1 Family 12

Family 12 Overview

2 experimentally studied proteins

38 sequences in Swiss-Prot

3,837 unique sequences in UniRef100

The Glutamyl-tRNA amido-transferase subunit D family, a group of achaeal enzymes that also contain the Class 1 asparaginase domain

Could also have l‑asparaginase activity and high affinity to l‑asparagine

Clan 4: Family 10, Family 11, Family 12, Family 13,

Reference Monomer1

mono1-12.png

Reference Structure

str1-12.png

Family 12 Motifs
(Q5JI77)

motifs1-12

Experimentally Studied Proteins

Fam ? Class - Clan - Family Alt ? Alternative historical name / classification AN ? UniProt accession number Name ? UniProt entry name, only given here for Swiss-Prot entries EC Organism Cell-Loc AAs Structure PDB Km i for Asn [mM] Vmax i for Asn [μmol/min/mg] Kcat i for Asn [s-1]
1-4-12 - O26802 GATD_METTH 6.3.5.- Methanothermobacter thermautotrophicus - 435 Hetero tetramer i Has additional domain. 2D6F - - -
1-4-12 - Q9V0T9 GATD_PYRAB 6.3.5.- Pyrococcus abyssi - 438 Hetero tetramer i Has additional domain. 1ZQ1 - - -

Swiss-Prot Sequences

Fam ? Class - Clan - Family Alt ? Alternative historical name / classification AN ? UniProt accession number Name ? UniProt entry name, only given here for Swiss-Prot entries EC Organism Cell-Loc AAs Structure PDB Km i for Asn [mM] Vmax i for Asn [μmol/min/mg] Kcat i for Asn [s-1]
1-4-12 - Q9Y9T8 GATD_AERPE 6.3.5.- Aeropyrum pernix - 427 - - - - -
1-4-12 - O29380 GATD_ARCFU 6.3.5.- Archaeoglobus fulgidus - 418 - - - - -
1-4-12 - B0R6H4 GATD_HALS3 6.3.5.- Halobacterium salinarum - 427 - - - - -
1-4-12 - Q9HP20 GATD_HALSA 6.3.5.- Halobacterium salinarum - 427 - - - - -
1-4-12 - Q18GL3 GATD_HALWD 6.3.5.- Haloquadratum walsbyi - 442 - - - - -
1-4-12 - Q8TR66 GATD_METAC 6.3.5.- Methanosarcina acetivorans - 424 - - - - -
1-4-12 - Q46GJ6 GATD_METBF 6.3.5.- Methanosarcina barkeri - 424 - - - - -
1-4-12 - Q12X65 GATD_METBU 6.3.5.- Methanococcoides burtonii - 415 - - - - -
1-4-12 - Q60331 GATD_METJA 6.3.5.- Methanocaldococcus jannaschii - 417 - - - - -
1-4-12 - Q8TV84 GATD_METKA 6.3.5.- Methanopyrus kandleri - 458 - - - - -
1-4-12 - A4FWR5 GATD_METM5 6.3.5.- Methanococcus maripaludis - 418 - - - - -
1-4-12 - A9AA46 GATD_METM6 6.3.5.- Methanococcus maripaludis - 418 - - - - -
1-4-12 - A6VGK5 GATD_METM7 6.3.5.- Methanococcus maripaludis - 418 - - - - -
1-4-12 - Q8PUM7 GATD_METMA 6.3.5.- Methanosarcina mazei - 425 - - - - -
1-4-12 - P61400 GATD_METMP 6.3.5.- Methanococcus maripaludis - 418 - - - - -
1-4-12 - A5UK11 GATD_METS3 6.3.5.- Methanobrevibacter smithii - 436 - - - - -
1-4-12 - A4YHH3 GATD_METS5 6.3.5.- Metallosphaera sedula - 439 - - - - -
1-4-12 - Q2NEH1 GATD_METST 6.3.5.- Methanosphaera stadtmanae - 438 - - - - -
1-4-12 - O26802 GATD_METTH 6.3.5.- Methanothermobacter thermautotrophicus - 435 Hetero tetramer i Has additional domain. 2D6F - - -
1-4-12 - A6UPR4 GATD_METVS 6.3.5.- Methanococcus vannielii - 426 - - - - -
1-4-12 - P61401 GATD_NANEQ 6.3.5.- Nanoarchaeum equitans - 392 - - - - -
1-4-12 - Q9V0T9 GATD_PYRAB 6.3.5.- Pyrococcus abyssi - 438 Hetero tetramer i Has additional domain. 1ZQ1 - - -
1-4-12 - Q8ZY04 GATD_PYRAE 6.3.5.- Pyrobaculum aerophilum - 417 - - - - -
1-4-12 - Q8U0X0 GATD_PYRFU 6.3.5.- Pyrococcus furiosus - 438 - - - - -
1-4-12 - O59132 GATD_PYRHO 6.3.5.- Pyrococcus horikoshii - 438 - - - - -
1-4-12 - Q97ZH5 GATD_SACS2 6.3.5.- Saccharolobus solfataricus - 444 - - - - -
1-4-12 - Q4J955 GATD_SULAC 6.3.5.- Sulfolobus acidocaldarius - 446 - - - - -
1-4-12 - C3N5E7 GATD_SULIA 6.3.5.- Sulfolobus islandicus - 445 - - - - -
1-4-12 - C4KH13 GATD_SULIK 6.3.5.- Sulfolobus islandicus - 445 - - - - -
1-4-12 - C3MPS1 GATD_SULIL 6.3.5.- Sulfolobus islandicus - 445 - - - - -
1-4-12 - C3MYR5 GATD_SULIM 6.3.5.- Sulfolobus islandicus - 445 - - - - -
1-4-12 - C3NHQ2 GATD_SULIN 6.3.5.- Sulfolobus islandicus - 445 - - - - -
1-4-12 - C3NE01 GATD_SULIY 6.3.5.- Sulfolobus islandicus - 445 - - - - -
1-4-12 - Q971W5 GATD_SULTO 6.3.5.- Sulfurisphaera tokodaii - 448 - - - - -
1-4-12 - Q9HJJ5 GATD_THEAC 6.3.5.- Thermoplasma acidophilum - 409 - - - - -
1-4-12 - Q5JI77 GATD_THEKO 6.3.5.- Thermococcus kodakarensis - 440 - - - - -
1-4-12 - A1RX40 GATD_THEPD 6.3.5.- Thermofilum pendens - 451 - - - - -
1-4-12 - Q979L8 GATD_THEVO 6.3.5.- Thermoplasma volcanium - 406 - - - - -

UniRef100 Sequences2

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Representative Sequence Alignment

Motifs Image

Additional Information

Clan 4 is an interesting clan of bacterial and archaeal l‑asparaginases. Some sequences display characteristics of both "type I" and "type II" l‑asparaginases and some are structurally unique.

Sequences in Family 12 are called glutamyl-tRNA(Gln) amidotransferase subunit D. These archaeal enzymes catalyse the transamidation from l‑glutamine to l‑glutamyl-tRNA(Gln) to form l‑glutaminyl-tRNA(Gln). The BLAST search results revealed some bacteria carry the gene coding this protein, too. In addition to the l‑asparaginase domain, they contain ~100 amino acid N-terminal residues. Together with glutamyl-tRNA(Gln) amidotransferase subunit E, they form the mature hetero dimer. l‑Asparagine can also be the substrate of this enzyme, acting as the amide donor.

1Varadi, M et al. AlphaFold Protein Structure Database in 2024: providing structure coverage for over 214 million protein sequences. Nucleic Acids Research (2024). Licensed under CC BY 4.0.

2Suzek, B.E. et al. UniRef: comprehensive and non-redundant UniProt reference clusters. Bioinformatics (2007). Licensed under CC BY 4.0. Added classification code to sequence headers.


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